Discovery and Proof-of-Concept Study of Nuclease Activity as a Novel Biomarker for Breast Cancer Tumors

Hernandez, Luiza I. and Araúzo-Bravo, Marcos J. and Gerovska, Daniela and Solaun, Ricardo Rezola and Machado, Isabel and Balian, Alien and Botero, Juliana and Jiménez, Tania and Zuriarrain Bergara, Olaia and Larburu Gurruchaga, Lide and Urruticoechea, Ander and Hernandez, Frank J. (2021) Discovery and Proof-of-Concept Study of Nuclease Activity as a Novel Biomarker for Breast Cancer Tumors. Cancers, 13 (2). p. 276. ISSN 2072-6694

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Abstract

Breast cancer is one of the most common pathologies diagnosed in the clinical practice. Despite major advancements in diagnostic approaches, there is no widely accepted biomarker in the clinical practice that can diagnose breast malignancy. Confirmatory diagnosis still relies on the pathological assessment of tissue biopsies by expert pathologists. Thus, there is an unmet need for new types of biomarkers and novel platform technologies that can be easily and robustly integrated into the clinic and that can assist pathologists. Herein, we show that nuclease activity associated to malignant tumors can be used as a novel biomarker in breast cancer, which can be detected via specific degradation of nucleic acid probes. In this study we have identified a set of three chemically modified nucleic acid probes that can diagnose malignancy in biopsy samples with high accuracy (89%), sensitivity (82%) and specificity (94%). This work represents a breakthrough for the potential clinical use of nuclease activity as biomarker, which can be detected via nucleic acids probes, for the clinical diagnosis of malignancy in breast tissue biopsies. This platform technology could be readily implemented into the clinic as adjunct to histopathological diagnostic.

Item Type: Article
Subjects: Scholar Eprints > Medical Science
Depositing User: Managing Editor
Date Deposited: 17 Jan 2023 06:47
Last Modified: 03 Oct 2024 04:35
URI: http://repository.stmscientificarchives.com/id/eprint/875

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